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Tissue Processing; Microtome & Staining










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Histology

Histotechnologists work in laboratories that look at tissues and organs with a light microscope. We do the preparatory work necessary so that the material can be examined by Pathologists.

 

The most common use is in health care, where it helps a pathologist determine whether a patient has cancer. A related specialty does similar preparatory work for examination of materials by the electron microscope.


The process used involves:

  • Record keeping to ensure there are no errors in identification.
  • Chemical preservation of the material to be examined.
  • Hardening and supporting the material with media, such as paraffin wax or plastic.
  • Cutting a very thin slice so that enough light can pass through to make individual components visible.
  • Staining components in contrasting colours to increase visibility and make examination easier.
  • Storing the finished product for future reference.




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